Fertilizare în Vitro

Longitudinal section of ovules cultured in vitro. (a) Inflorescence segment culture. Note the prominent egg cell (arrow) with one synergid and polar nucleus. All other cells of the embryo sac degenerated(b) Parthenogenetic 3-celled proembryo (arrow). Note the first periclinal division of the egg, resulting in an upper cell and a lower cell. The lower cell has divided into daughter cells(c) An ovule at the time of excised ovary culture. A 26–32-celled globular proembryo (arrow) has developed at the micropylar end(d) An ovule containing the gynogenic proembryo and autonomous endosperm formed by division of thepolar nucleus or secondary nucleus. Sections were taken 3 days after isolated ovary culture.(a) Ovary three weeks after isolated ovary culture.Note the emergence of hypocotyl and radicle regions from the ovary(b) Gynogenic embryo 4 weeks after isolated ovaryculture. The cotyledons and radicle are very clear. Also notethe shrivelled ovary wall attached to the gynogenic plant.