Nicotina

O metoda simpla si rapida de electroforeza capilara (CE) a fost dezvoltata pentru o cantitate de nicotina din produse comerciale din tutun. Metoda implica rulare de 6 min la 30 kV, folosind 50 mM solutie tampon de fosfat (pH 2,5), folosit ca standard intern,

si o detectie UV la 260 nm. Nicotina a fost extrasa din produsele din tutun in <15 min. Recuperari de la extractele tinte au fost >95%, si eficienta de extractie a apei, HCl 1M, acidului acetic 1M, solutiei tampon fosfat 5mM (pH 2,5) si trietanololului amino 1% au fost similare. Concentratiile de nicotina in 67 probe de tigari, trabucuri au variat intre 0.37 si 2.96% (w / w). O metoda de analiza stabilita GC-MS utilizand metoda de extractie cu toluen a dat in mod constant valori mai mici de nicotina decat metoda de CE. Dovezi experimentale sugereaza ca acest lucru se datoreaza extractiei insuficiente de nicotina cu toluen.

Gorrod, J.W., & Jacob III, P. (1999) Analytical Determination of Nicotine and Related Compounds and Their Metabolites, Elsevier Science B.V., Amsterdam, The Netherlands;

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Yang, S.S., Smetena, I., & Goldsmith, A.I. (1996) J. Chromatogr. A 746, 131-136;

Siegmund, B., Leitner, E., & Pfannhauser, W. (1999) J. Chromatogr. A 840, 249-260;

Siegmund, B., Leitner, E., & Pfannhauser, W. (1999) J. Agric. Food Chem. 47, 3113-3120;

Federal Trade Commission at www.ftc.gov/bcp/conline/pubs/alerts/smokealrt.htm.